

陳洪淵
個性化簽名
- 姓名:陳洪淵
- 目前身份:
- 擔任導師情況:
- 學位:
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學術頭銜:
博士生導師, 中國科學院院士
- 職稱:-
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學科領域:
分析化學
- 研究興趣:
陳洪淵,教授,博士生導師,中國科學院院士。1961年畢業于南京大學化學系,1981-1984年為德國Mainz大學訪問學者。南京大學化學化工學院教授、博士生導師。2001年當選為中科院院士?,F任南京大學教學委員會副主任,南京大學分析科學研究所和化學生物學研究所所長。目前兼任國家教育部科技委委員、化學化工學部主任;中國科學院化學學部常委;國家自然科學獎勵委員會成員、評委;國家自然科學基金委咨詢委員會委員,學科、學部評委;中國化學會理事,分析化學委員會副主任;江蘇省化學化工學會理事長,江蘇省質量協會與質量學會副理事長,江蘇省測試協會與測試學會副理事長;全國儀器儀表學會儀器委員會委員,全國化學傳感器專業委員會副主任等?!陡叩葘W?;瘜W學報》和《Chem. Res. in Chin. Univ.》副主編,《中國化學(英)》、《中國科學(中,英)》等刊編委。曾任《國際純粹與應用化學聯合會(IUPAC)》電化學委員會國家代表。他所主持的科研組已經在國內外重要刊物發表論文450多篇,論文被他人引用2000多次; 合編著譯書8冊,專利5項。 迄今已指導博士后8名, 培養博士42名,碩士33名。此外,他還曾榮獲全國科學大會獎、教育部中國高校自然科學一等獎、國家自然科學三等獎、國家教委及教育部和省科技進步三等獎、省人才培養獎等獎項。
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主頁訪問
9480
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關注數
0
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成果閱讀
563
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成果數
20
陳洪淵, Yi Xiao, Huang-Xian Ju, Hong-Yuan Chen*
Analytica Chimica Acta 391(1999)73-82,-0001,():
-1年11月30日
A novel strategy to construct a sensitive amperometric sensor of H2O2 was described. Horseradish peroxidase (HRP) wassuccessfully immobilized on nanometer-sized Au colloids, which were supported by thiol-tailed groups of cysteaminemonolayer. The thiol-tailed groups were formed through the covalent binding of glutaraldehyde on a cysteamine-modifedgold electrode. With the aid of the catechol mediator in the solution, HRP-labeled Au colloids displayed excellentelectrocatalytical response to the reduction of H2O2, which increased with decreasing size of Au colloids. The sensorsresponded to H2O2 in the concentration range of 0.39 mM-0.33 mM, and reached 95% of the steady-state current in less than5 s with the detection limit of 0.15 mM. The response showed a Michaelis-Menten behavior at larger H2O2 concentrations.The KappM values for the sensors based on different sized HRP-labeled Au colloids (colloid A-D) were found to be 0.11, 0.094,0.054 and 0.064 mM, respectively. The low KappM values demonstrated that HRP immobilized on Au colloids exhibited a highaf
Self-assembled monolayers, HRP-labeled Au colloids, Cysteamine, Hydrogen peroxide, Sensors, Electrochemical sensors
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陳洪淵, Xiao Yi, Ju Huang-Xian, and Chen Hong-Yuan
,-0001,():
-1年11月30日
Direct electron transfer of immobilized horseradishperoxidase on gold colloid and its application as a biosensorwere investigated by using electrochemicalmethods. The Au colloids were associated with a cysteaminemonolayer on the gold electrode surface. A pairof redox peaks attributed to the direct redox reaction ofhorseradish peroxidase (HRP) were observed at theHRP/Au colloid/cysteamine-modi?ed electrode in 0.1 Mphosphate buffer (pH 7.0). The surface coverage of HRPimmobilized on Au colloid was about 7.6×10-10 mol/cm2.The sensor displayed an excellent electrocatalytic responseto the reduction of H2O2 without the aid of anelectron mediator. The calibration range of H2O2 was 1.4mMto 9.2mMwith good linear relation from 1.4mMto 2.8mM. A detection limit of 0.58 mM was estimated at asignal-to-noise ratio of 3. The sensor showed good reproducibilityfor the determination of H2O2. The variationcoef?cients were 3.1 and 3.9% (n=10) at 46 mM and 2.8mM H2O2, respectively. The response showed a Michaelis-Menten behavior at higher H2O2 concentrations. TheKMapp value for the H2O2 sensor was found to be 2.3 mM.
horseradish peroxidase, direct electrochemistry, Au colloid, cysteamine, biosensor,, self-assembled monolayers.,
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陳洪淵, Dong-Mei Zhou, Huang-Xian Ju, Hong-Yuan Chen*
Journal of Electroanalytical Chemistry 408(1996)219-223,-0001,():
-1年11月30日
A chemically modified microelectrode has been prepared successfully by means of electrodepositing nickel hexacyanoferrate(II-III)on a microdisk platinum electrode. This modified electrode could catalyze the oxidation of dopamine and, moreover, eliminate theelectrode poisoning caused by the electrochemical polymerization of dopamine at the bare platinum electrode. However, the oxidation ofascorbic acid could also be catalyzed at this modified electrode, thus interfering with the electrochemical response of dopamine. When themodified electrode was further covered with Nation
Microelectrode, Double-film modified electrode, Dopamine, Nation
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陳洪淵, Hai-Ying Gu, Ai-Min Yu, Hong-Yuan Chen*
Journal of Electroanalytical Chemistry 516(2001)119-126,-0001,():
-1年11月30日
Hemoglobin (Hb) was immobilized successfully on nanometer-sized gold colloid particles associated with a cysteaminemonolayer on a gold electrode surface, and was characterized by atomic force microscopy (AFM), UV–vis spectroscopy(UV–vis), surface enhanced Raman spectroscopy (SERS) and electrochemical impedance spectroscopy (EIS). The immobilizedHb was shown to keep its biological activity well. Direct electron transfer (eT) between Hb and the modified electrode wasachieved without the aid of any electron mediator. In pH 7.2 phosphate buffer solution, the formal potential (E°[1]) of Hb was?0.051 V (vs. SCE) and the eT rate constant was 0.49 s?1. The average surface coverage of Hb immobilized on the gold colloidwas about 6.71×10?11 mol cm?2. The immobilized Hb displayed the features of a peroxidase and gave an excellentelectrocatalytic response to the reduction of H2O2. The Michaelis–Menten constant (Km) was 1.2×10?4 M. The currents wereproportional to the H2O2 concentration from 3.6×10?7 to 8.6×10?4 M and the detection limit was as low as 1.2×10?7 M(S/N=3).
Hemoglobin, Direct electron transfer (, eT), , Gold colloid, Self-assembled monolayer, Hydrogen peroxide
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陳洪淵, Chen-Xin Cai*, Huang-Xian Ju, Hong-Yuan Chen
,-0001,():
-1年11月30日
A stable electroactive thin film of cobalt hexacyanoferrate (CoHCF) was electrochemically deposited on the surface of a microbandgold electrode. The cyclic voltammograms of CoHCF film indicate the presence of two redox peaks corresponding to the hexacyanofer-rate(ll/lII) redox couple. The electrochemical behaviour of CoHCF is related to the concentration of supporting electrolyte andcounter-ions. The modified electrode shows excellent electrocatalytic activity towards the oxidation of reduced nicotinamide adeninedinucleotide (NADH) in phosphate buffer solution (pH 7.0), with an overpotential ca.310-370 mV lower than that of the bare microbandgold electrode. The catalytic peak current is proportional to NADH concentration in the range 0.5-6.0 mM with a correlation coefficientof 0.98. The catalytic rate constant of the modified electrode for the oxidation of NADH is determined using a rotating-disc electrode.The mechanism of the oxidation of NADH catalysed by the electrode is discussed.
Microelectrodes, Catalytic methods, Electrocatalytic oxidation, NADH, Cobalt hexacyanoferrate
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陳洪淵, Chen-Xin Cai, Huang-Xian Ju, Hong-Yuan Chen*
,-0001,():
-1年11月30日
Nickel hexacyanoferrate films have been deposited on the surface of a microband gold electrode by cyclic voltammetry.Cyclic voltammograms of the resulting nickel hexacyanoferrate films indicate the presence of a couple of redox peaks whichcorrespond to the hexacyanoferrate(II/III) redox couple. The nickel hexacyanoferrate modified microband gold electrodeshows an excellent electrocatalytic activity for oxidation of reduced nieotinamide adenine dinueleotide (NADH) inphosphate buffer solution (pH 7.0), with an overpotential ca. 340mV lower than that of the bare electrode. The steady-statecurrent increases linearly with NADH concentration in the range of 0.5-8.0 mmolx. The catalytic rate constant of themodified electrode for the oxidation of NADH is determined using a rotating disk electrode. The further experimental resultsshow that the rate constant decreases with increasing NADH concentration. The mechanism of the oxidation of NADHcatalyzed by the nickel hexacyanoferrate modified gold electrode is discussed.
Catalytic methods, Microelectrodes, Electrocatalytic oxidation, NADH, Nickel hexacyanoferrate
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【期刊論文】Interfacing cytochrome c to electrodes with a DNA-carbon nanotube composite film
陳洪淵, Gang Wang, Jing-Juan Xu, Hong-Yuan Chen*
Electrochemistry Communications 4(2002)506-509,-0001,():
-1年11月30日
Multi-walled carbon nanotube (MWCNT) is successfully immobilized on the surface of platinum electrode by mixing with DNA.The DNA/MWCNT modified electrodes are characterized by electrochemical impedance spectroscopy and cyclic voltammetry.Further research indicates that cytochrome c can strongly adsorbed on the surface of the modified electrode, and forms an approximatemonolayer. The immobilized MWCNT can promote the redox of horse heart cytochrome c which gives reversible redoxpeaks with a formal potential of 81 mV vs SCE.
DNA molecular, Carbon nanotube, Electrochemical impedance spectroscopy, Cytochrome c
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【期刊論文】Voltammetric studies of the interaction of methylene blue with DNA by means of β-cyclodextrin
陳洪淵, Guang-Chao Zhao, Jun-Jie Zhu, Jing-Jie Zhang, Hong-Yuan Chen*
Analytica Chimica Acta 394(1999)337-344,-0001,():
-1年11月30日
The formation of an inclusion complex of methylene blue with b-cyclodextrin is testi?ed by voltammetry and spectroscopyand its stable constant is determined to be 4.4×103M-1. The interactions of methylene blue and the inclusion complex withDNA have been investigated by means of voltammetry. The binding constants of methylene blue and the inclusion complex toDNA are obtained through the changes of peak currents and their values are 1.1×104M-1 and 7.2×103M-1, respectively.The experimental results reveal that the inclusion complex does not decompose when it interacts with DNA, and bcyclodextrinitself does not interact with DNA.Their binding numbers (n) are 1 both methylene blue and the inclusioncomplex. According to the experimental data, it can be inferred that the binding model of methylene blue to DNA may be`electrostatic binding' under our experimental conditions. This can clearly explain the experimental phenomena.
Methylene blue, Inclusion complex, Interaction, DNA, Voltammetry
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陳洪淵, Hua Lin, Dan-Ke Xu, Hong-Yuan Chen*
Journal of Chromatography A, 760(1997)227-233,-0001,():
-1年11月30日
Simultaneous determination of purine bases, ribonucleosides and ribonucleotides was achieved by coupling capillary electrophoresis (CE) with wall-jet amperometric detection. A 200um diameter copper disk electrode was applied at working potential, +0.65 V vs. saturated calomel electrode. The current response of high sensitivity and stability was obtained in strong basic solutions which were suitable for satisfactory CE separations. The calibration curve was linear over 2-3 orders of magnitude and the limits of detection for adenine, guanine, xanthine, uric acid, adenosine, guanosine, adenosine-5-monophosphate and guanosine-5'-monophosphate were below 9 fmol (S/n=3). The use of this method for the separation and detection of compounds present in human plasma samples was reported.
Detection,, electrophoresis: Electrodes, Purines, Ribonucleosides: Ribonucleotides
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陳洪淵, Gang Wang a, Jing-Juan Xu a, Hong-Yuan Chen a, *, Zu-Hong Lu b
Biosensors and Bioelectronics 18(2003)335-343,-0001,():
-1年11月30日
A new type of sol /gel/organic hybrid composite material based on the cross-linking of natural polymer chitosan with (3-aoryloxypropyl) dimethoxymethylsilane was developed for the fabrication of an amperometric H2O2 biosensor. The composite filmwas used to immobilize horseradish peroxidase (HRP) on a gold disk electrode. The properties of sol-gel/chitosan and sol-gel/chitosan-HRP films have been carefully characterized by atomic force microscopy and Fourier transform infrared. By usingfluorescent label, a protein density on sol /gel/chitosan has been calculated to be 3.14×1012 molecules cm 2. With the aid ofcatechol mediator, the biosensor had a fast response of less than 2 s with linear range of 5.0×10-9-1.0×10-7 mol-1 and adetection limit of 2×10-9 mol-1. Its current response shows a typical Michaelis-Menten mechanism. The apparent Michaelis-Menten constant KappM is found to be 1.30 mmol 1. The activation energy for enzymatic reaction is calculated to be 8.22 kJ mol 1.The biosensor retained approximately 75% of its original activity after about 60 days of storage in a phosphate buffer at 4℃.
Sol-gel/, chitosan composite film, Horseradish peroxidase, Biosensor, Hydrogen peroxide
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